Journal: Experimental and Therapeutic Medicine

Article Title: Scutellaria barbata D. Don inhibits growth and induces apoptosis by suppressing IL-6-inducible STAT3 pathway activation in human colorectal cancer cells

doi: 10.3892/etm.2015.2692

Figure Lengend Snippet: Effect of EESB on the HT-29 cell cycle. Cells were pretreated with various concentration of EESB for 1 h, followed by stimulation with 10 ng/ml IL-6 for 24 h. (A) Cells were stained with propidium iodide and analyzed by fluorescence-activated cell sorting. The proportion of DNA in the S phase was calculated using ModFit LT version 3.0 software. (B) Quantification of fluorescence-activated cell sorting analysis. The data shown are averages with standard deviation from 3 independent experiments. #P<0.05 vs. cells treated with IL-6 but not EESB. EESB, ethanol extract of Scutellaria barbata D. Don; IL-6, interleukin-6.

Article Snippet: The HT-29 cell cycle progression was determined through flow cytometric analysis using a propidium iodide (PI) staining cell cycle assay kit (BD Biosciences, Franklin Lakes, NJ, USA).

Techniques: Concentration Assay, Staining, Fluorescence, FACS, Software, Standard Deviation

Journal: Experimental and Therapeutic Medicine

Article Title: Scutellaria barbata D. Don inhibits growth and induces apoptosis by suppressing IL-6-inducible STAT3 pathway activation in human colorectal cancer cells

doi: 10.3892/etm.2015.2692

Figure Lengend Snippet: Effect of EESB on HT-29 cell apoptosis. Cells were pretreated with various concentration of EESB for 1 h, followed by stimulation with 10 ng/ml IL-6 for 24 h. (A) Cells were collected and stained with Annexin V/PI, followed by fluorescence-activated cell sorting analysis. Double-negative stained cells indicate the live cell population; Annexin V-positive/PI-negative stained cells and Annexin V/PI double-positive stained cells represent early and late apoptosis, respectively; Annexin V-negative and PI-positive stained cells show dead cells. (B) Quantification of fluorescence-activated cell sorting analysis. The data shown are averages with standard deviation from 3 independent experiments. #P<0.05 vs. cells treated with IL-6 but not EESB. EESB, ethanol extract of Scutellaria barbata D. Don; IL-6, interleukin-6; UL, upper left; UR, upper right; LR, lower right; LL, lower left; PI, propidium iodide; FITC, fluorescein isothiocyanate.

Article Snippet: The HT-29 cell cycle progression was determined through flow cytometric analysis using a propidium iodide (PI) staining cell cycle assay kit (BD Biosciences, Franklin Lakes, NJ, USA).

Techniques: Concentration Assay, Staining, Fluorescence, FACS, Standard Deviation

Journal: Journal of Cellular and Molecular Medicine

Article Title: Long non‐coding RNA AFAP1‐AS1/miR‐320a/RBPJ axis regulates laryngeal carcinoma cell stemness and chemoresistance

doi: 10.1111/jcmm.13707

Figure Lengend Snippet: AFAP1‐AS1 enhances cisplatin resistance in laryngeal carcinoma cells. A, HEp‐2 was treated with 4 μmol L −1 cisplatin. Expression of AFAP1‐AS1 was analysed at various times (0, 6, 12, 18, 24, and 30 h) by qRT‐PCR. * P < .05, ** P < .01, compared with 0 h. B, AFAP1‐AS1 silenced HEp‐2 cells were cultured in 96‐well plates. Cell viability was analysed by CCK8 assay under treatment with various concentration of cisplatin (0, 2, 4, 8, 16 and 32 μmol L −1 ). ** P < .01, compared with control siRNA transfected cells. C, Apoptosis assays in AFAP1‐AS1 silenced HEp‐2 cells under 8 μmol L −1 cisplatin treatment. ** P < .01, compared with control siRNA transfected cells. D, Cisplatin‐resistant HEp‐2 cell lines (HEp‐2/R) were established. Cell viability assays were performed in HEp‐2 and HEp‐2/R cells under various concentrations of cisplatin treatment. * P < .05, ** P < .01, compared with HEp‐2 cells. E, Expression of AFAP1‐AS1 in HEp‐2 and HEp‐2/R cells was analysed by qRT‐PCR. ** P < .01, compared with HEp‐2 cells

Article Snippet: We conducted nuclear DAPI staining to access cell apoptosis using Cell Apoptosis DAPI Detection Kit (GenScript, Piscataway, NJ) according to manufacturer's instructions.

Techniques: Expressing, Quantitative RT-PCR, Cell Culture, CCK-8 Assay, Concentration Assay, Control, Transfection

Journal: Journal of Cellular and Molecular Medicine

Article Title: Long non‐coding RNA AFAP1‐AS1/miR‐320a/RBPJ axis regulates laryngeal carcinoma cell stemness and chemoresistance

doi: 10.1111/jcmm.13707

Figure Lengend Snippet: miR‐320a reduces stemness and cisplatin resistance in laryngeal carcinoma cells. A, HEp‐2 cell morphology of parental cells and stemness‐enriched cell spheres (left) and corresponding miR‐320a expression (right). ** P < .01, compared with parental cells. B, Expression of miR‐320a in miR‐320 overexpression HEp‐2 cells by qRT‐PCR. *** P < .001, compared with control miRNA transfected cells. C, Expression of stemness‐associated genes in miR‐320a overexpression HEp‐2 cells. Gene expression was analysed by qRT‐PCR. * P < .05, ** P < .01, compared with control miRNA transfected cells. D, Number of tumour spheres in miR‐320a overexpression HEp‐2 cells. ** P < .01 compared with control miRNA transfected cells. E, miR‐320a overexpression HEp‐2 cells were cultured in 96‐well plates. Cell viability was analysed using CCK8 assays under various concentrations of cisplatin (0, 2, 4, 8, 16 and 32 μmol L −1 ). ** P < .01, compared with control miRNA transfected cells. F, Apoptosis assay in miR‐320a overexpression HEp‐2 cells under 8 μmol L −1 cisplatin treatment. ** P < .01, compared with control miRNA transfected cells. G, Expression of miR‐320a in HEp‐2 and HEp‐2/R cells was analysed by qRT‐PCR. ** P < .01, compared with HEp‐2 cells

Article Snippet: We conducted nuclear DAPI staining to access cell apoptosis using Cell Apoptosis DAPI Detection Kit (GenScript, Piscataway, NJ) according to manufacturer's instructions.

Techniques: Expressing, Over Expression, Quantitative RT-PCR, Control, Transfection, Gene Expression, Cell Culture, Apoptosis Assay

Journal: Journal of Cellular and Molecular Medicine

Article Title: Long non‐coding RNA AFAP1‐AS1/miR‐320a/RBPJ axis regulates laryngeal carcinoma cell stemness and chemoresistance

doi: 10.1111/jcmm.13707

Figure Lengend Snippet: AFAP1‐AS1 regulates laryngeal carcinoma cells through miR‐320a/ RBPJ. A, Expression of RBPJ mRNA in AFAP1‐AS1 silenced, miR‐320a inhibition (miR‐320a‐in) and AFAP1‐AS1 silenced plus miR‐320a inhibition HEp‐2 cells by qRT‐PCR. * P < .05, compared with control cells. B, Expression of RBPJ protein in AFAP1‐AS1 silenced, miR‐320a inhibition and AFAP1‐AS1 silenced plus miR‐320a inhibition HEp‐2 cells by Western blot. C, Expression of RBPJ mRNA in AFAP1‐AS1 silenced, RBPJ and AFAP1‐AS1 silenced plus RBPJ HEp‐2 cells by qRT‐PCR. * P < .05, compared with control cells. D, Expression of RBPJ mRNA in AFAP1‐AS1 silenced, RBPJ and AFAP1‐AS1 silenced plus RBPJ HEp‐2 cells by Western blot. E, Number of tumour spheres in AFAP1‐AS1 silenced, RBPJ and AFAP1‐AS1 silenced plus RBPJ HEp‐2 cells. * P < .05 compared with control cells. F, Apoptosis assay in AFAP1‐AS1 silenced, RBPJ and AFAP1‐AS1 silenced plus RBPJ HEp‐2 cells under 8 μmol L −1 cisplatin treatment. * P < .05, compared with control cells

Article Snippet: We conducted nuclear DAPI staining to access cell apoptosis using Cell Apoptosis DAPI Detection Kit (GenScript, Piscataway, NJ) according to manufacturer's instructions.

Techniques: Expressing, Inhibition, Quantitative RT-PCR, Control, Western Blot, Apoptosis Assay

Journal: ACS Omega

Article Title: Taurine-Conjugated Mussel-Inspired Iron Oxide Nanoparticles with an Elongated Shape for Effective Delivery of Doxorubicin into the Tumor Cells

doi: 10.1021/acsomega.0c01747

Figure Lengend Snippet: Cells apoptosis determined by Annexin V and PI staining of (A) absolute control, (B) T-pDA-Fe 3 O 4 , (C) free drug, DOX, and (D) Dox-loaded T-pDA-Fe 3 O 4 .

Article Snippet: Annexin V-FITC detection kit with PI staining for cell apoptosis was obtained from BD biosciences (Franklin Lakes, NJ, USA).

Techniques: Staining